FIG. 6.
Viral DNA replication in the presence and absence of Olo or Rosco. HEL cells (9 × 105) in 60-mm dishes (320 cells/mm2) were infected with 2.5 PFU of HSV-1 per cell in the presence of 75 μM Olo (OLO) or 40 μM Rosco (ROSCO) or in the absence of a drug (CONTROL). DNA was extracted from infected cells at 1, 3, 6, 9, 12, and 18 hpi and from mock-infected cells (MI). Five micrograms of total DNA was blotted onto a nitrocellulose membrane, UV cross-linked, and hybridized to labeled HSV-specific riboprobes, and the membrane was exposed in a PhosphorImager (A). The signal hybridized to each slot was quantitated and corrected for loading as measured by GAPDH hybridization, and the amount of viral DNA in each sample at the indicated time after infection was plotted (B). The insert in panel B shows the amounts of viral DNA detected during the interval between 1 and 3 hpi on an expanded scale.