FIG. 8.
Hepatoma cells expressing IRNA inhibit translation of the PV-HCV chimera. Approximately 3.5 × 105 monolayer hepatoma cells (Huh-7) (lanes 1 and 3) or IRNA-expressing hepatoma cells (lanes 2 and 4) were infected with approximately 3.75 × 103 PFU of either PV-HCV (lanes 1 and 2) or PV-EMCV (lanes 3 and 4) chimera. After 24 h of infection, cells were labeled with [35S]methionine. In vivo-labeled proteins were immunoprecipitated with anti-PV capsid antibody and analyzed on an SDS–14% polyacrylamide gel. The positions of the PV capsid proteins are indicated on the left. Numbers at the right refer to the approximate molecular masses (in kilodaltons) of the 14C-labeled protein markers (Amersham).