TABLE 1.
Virus titers
| Chimera | Virus titer (PFU/ml)a
|
||
|---|---|---|---|
| Huh-7 | Hepatoma-IRNA | Hepatoma-ribozyme | |
| PV-HCV | 5.9 × 108 | 1.0 × 106 | 5.2 × 108 |
| PV-EMCV | 1.8 × 108 | 0.8 × 108 | 1.6 × 108 |
a
3 × 105 cells in 30-mm-diameter plates were infected with 150 μl of PV-HCV or PV-EMCV chimera (2.5 × 104 PFU/ml) for 24 h. Cell extracts were prepared from infected cells, and virus titers were determined by infecting 2 × 106 HeLa cells and counting plaques after 48 h of infection. Each titer is an average of three experiments.