Fig. 7 |. Inflammatory and cytotoxic human exT_reg genes overexpressed in individuals with coronary artery disease.

a, Genes that were significantly upregulated in human exT_reg cells compared to T_reg cells (2,803 genes) in the human bulk RNA-seq data (Fig. 3) were intersected with the genes present in the published human scRNA-seq panel (Fig. 2). Donor details for human bulk RNA-seq are in Supplementary Table 13 and those for scRNA-seq are in ref. 21. b, Mean expression of genes in CD16⁺CD56⁺ exT_reg cells from the scRNA-seq dataset that were significantly increased in CAD⁺ non-diabetic (brown circles in top, n = 7) or diabetic (red squares in bottom, n = 11) individuals in comparison to control CAD⁻ non-diabetic (black circles, n = 12) individuals. Results are shown as the mean ± s.e.m. Each point represents data from exT_reg cells from an independent donor. Statistical comparisons were done using a two-tailed Mann–Whitney U test. Top, **P = 0.0064 (CNOT2), *P = 0.0414 (CCL4), **P = 0.009 (IL18RAP), *P = 0.0361 (KLRG1), *P = 0.0163 (KLRC1), *P = 0.0371 (LYN), **P = 0.0095 (SAMD3), *P = 0.0395 (SYNE1). Bottom, *P = 0.0126 (CCL5), *P = 0.0354 (FGFBP2), *P = 0.0373 (ITGA4), *P = 0.018 (ITGAM), *P = 0.031 (KLRB1), *P = 0.032 (KLRC1). c, log₂FC and adjusted P values of a DEG analysis (based on a two-tailed Wald test with Benjamini–Hochberg P-value adjustment) between exT_reg cells and T_reg cells for the 13 CAD-relevant exT_reg genes from b.