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. 2023 Dec 15;22(5):1352–1371. doi: 10.1111/pbi.14271

Figure 8.

Figure 8

OsWRKY45 genetically acts upstream of OsGELP77. (a) OsGELP77 expression in OsWRKY45‐OE and oswrky45 mutant. (b) DNA binding activity assay of OsWRKY45 by EMSA assay. (c) Binding assay of OsWRKY45 to the promoter of OsGELP77 by ChIP‐qPCR in OsWRKY45:GFP plants using the anti‐GFP antibody. Anti‐GFP antibody was used for immunoprecipitation and IgG acted as a control. The blue capital letters indicate the intact W‐box, the red capital letters represent the mutated W‐box. (d) Activity assay of OsWRKY45 in regulating OsGELP77 expression. (e) JA contents in the leaves of transgenic plants and the corresponding background. (f) Responses of the transgenic plants and the corresponding background to Xoo strain PXO99. Plants were inoculated with Xoo at the booting stage. (g) FPKM values of OsWRKY45‐1 and OsWRKY45‐2 in rice leaf. (h) Responses of rice varieties harbouring OsGELP77 Hap3 and OsWRKY45‐1 or OsWRKY45‐2 to Xoo strain PXO99. Plants were inoculated with Xoo at the booting stage. Data represent means ± SD. n = 3 (a, c, d, e), n = 30 (f). Asterisks in (a, c, d) indicate significant differences between transgenic plants and WT or control determined by two‐tailed Student's t‐test at **P < 0.01 or *P < 0.05. The different letters above each bar in (e, f, g, h) indicate statistically significant differences, as determined by one‐way ANOVA analysis followed by Tukey's multiple test (P < 0.05).