Fig. 3.

Dependence of in vitro activation of CYP46A1 on the concentration of EFV (A), its mono- (B) and dihydroxylated (C) metabolites. The Y-axis is identical for all three graphs and represents CYP46A1 activity as nanomoles of 24-hydoxycholsterol (24HC) formed per nmole of CYP46A1 per minute. The results are the mean ±S.D. of the measurements from the three independent experiments. Statistically significant differences between (S)-EFV versus (R)-EFV were assessed by two-way ANOVA with Bonferroni multiple comparisons. No significant differences were found between the (S) enantiomer versus racemate of the same EFV metabolite * P ≤ 0.05.