TABLE 3.

Summary of incubation conditions and positive controls used in the P450-Glo assays

Enzyme	Inhibitor	Dilution Format	Inhibitor Concentration	Incubation Conditions
CYP3A4	Ketoconazole	16 concentrations/2-fold dilution in duplicates	57 µM to 1.8 nM	1 h/RT
CYP2C9	Sulfaphenazole		57 µM to 1.8 nM	1 h/ 37°C/ 0.4% BSA
CYP2D6	Quinidine		1.4 µM to 0.04 nM	1 h/RT