Fig. 5. (a) Fluorescence confocal imaging (I and II, scale bar: 10 μm) and intracellular fluorescence intensity switching (III) of HeLa cells incubated with DS@M (10 μM), upon alternate irradiation with 475/560 nm light (excitation: 475 nm; emission: 500–650 nm; irradiation with 475 nm: 10 mW cm2; irradiation with 560 nm: 30 mW cm−2). (b) Fluorescence lifetime imaging (I and II, scale bar: 10 μm) and FLIM histogram (III) of HeLa cells incubated with DS@M (10 μM), upon alternate irradiation with 475/560 nm light. Scale bar: 10 μm; color bar: fast lifetimes (ns). (c) Super-resolution STORM (right) and conventional wide-field (left) images of the HeLa cell (I, scale bar: 4 μm). Calculation of FWHM in HeLa cells of the STORM image and wide-field image, respectively (II and III).
