Table 4.

Next-generation sequencing of either primary or metastatic tissue from patients with AMPC.

Patient ID	Tissue Source (1=primary, 2=metastatic)	Germline Mutation	Somatic Mutations
2	1	Not tested	TP53 (p.R273C)
2	2	Not tested	TP53 (p.R273C), TMPRSS2-ERG fusion, AR amplification
3	1	MSH2	Biallelic mutation in MSH2 (exon 3–16 dletion, with associated LOH); positive for microsatellite instability; TP53 (p.R273C), hypermutation with TMB of 20 mutations/Mb
5	1	None	TMPRSS2-ERG rearrangement
9	2	Unknown	BRCA2 deletions in exons 3–11 with LOH; PTEN deletion (exons 6- 8); FOXA1 in- frame insertion (c.797ins24); TP53 (p.C2777Gfs*63); APC (p.G520X) with LOH; AR amplification
11	2	Not Tested	None
13	2	Not Tested	TMPRSS2-ERG rearrangement