Extended Data Fig. 9. Gating strategy to analyse wound immune cell dynamics in diabetic mice as well as cytokine and protease levels after eCGRP treatment.
a, Gating strategy to analyse myeloid cells skin and muscle post-injury. Flow cytometry dot plots representing the step by step (1–6) gating strategy to identify neutrophil (CD11b+, Ly6G+, F4/80–) and macrophage populations (CD11b+, F4/80+, Ly6G–). MFI is the geometric-mean of fluorescence intensity. b, Saline or eCGRP was delivered on Leprdb/db skin wounds or in quadricep volumetric muscle loss defect via a fibrin hydrogel. The levels of CCL2, IL-1β, CXCL2, MMP-2, and MMP-9 in injured tissues were quantified by ELISA (n = 8 for skin, n = 4 for muscle). Data are plotted in box plots showing median (central line) and IQR (bounds). Whiskers show min. to max. range. Dots represent individual injuries. Two-way ANOVA with Bonferroni post hoc test for pair-wise comparisons. P values are indicated.