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. 2024 Mar 27;628(8008):604–611. doi: 10.1038/s41586-024-07237-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a, Analysis of neutrophil and monocyte/macrophage (Mo/Mϕ) populations by flow cytometry during tissue healing. Geometric mean of fluorescence intensity (MFI) of CD206 in macrophages was used to assess M2-like polarization. Data are plotted in kinetic line plots showing mean ± s.e.m. Skin: day 0, day 6 and day 10, n = 20; day 3, n = 22; and day 14, n = 12. Muscle: day 0, n = 16; day 3, n = 20; day 6, day 10, n = 18; and day 14, n = 10. b–e, Neutrophils and macrophages were treated with saline (PBS, 0 nM CGRP) or CGRP (1 or 20 nM). Results are expressed as fold change over the PBS (0 nM CGRP) group. b, Transwell migration towards CXCL1 or CCL2 with or without CGRP (n = 6–8). c, Cell death in response to CGRP and TNF plus IL-1 (neutrophils, n = 7; macrophages, n = 6). d, Macrophage efferocytosis of neutrophils after CGRP treatment with or without TNF/IL-1 (n = 4). e, Macrophage polarization determined via CD206 and arginase-1 protein expression following CGRP and IL-4/IL-13 or IL-10 treatment (n = 6). f, tdTomato⁺ bone marrow cells were administered systemically on day 2 post-injury. Fold change of tdTomato⁺ neutrophils and monocytes/macrophages in injured tissues on day 3 post-injury was assessed by flow cytometry. For efferocytosis, dead or dying tdTomato⁺ neutrophils were injected in skin wound borders on day 3 post-injury. Fold change of tdTomato⁺ endogenous monocytes/macrophages was assessed by flow cytometry 30 min post-injection (n = 6). g, Death of CD11b⁺ cells 3 days post-injury, assessed by TUNEL assay on injured tissue sections (n = 6). Boxes show median (centre line) and interquartile range (edges), whiskers show the range of values. Dots represent independent experiments. a–e, Two-way ANOVA with Bonferroni post hoc test for pairwise comparisons. f,g, Two-tailed Student’s t-test. P values are indicated. NS, not significant.

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