TABLE 4.
Detection of viral DNA by PCR in organs of persistently infected mice
| Wk post- irradiation | Virus | No. of mice positive/no. assayed (%)a
|
||
|---|---|---|---|---|
| Brain | Kidney | Spleen | ||
| 0 | wt | 3/3 | 3/3 | 2/3 |
| dlE102 | ND | ND | ND | |
| dlE105 | 0/4 | 0/4 | 0/4 | |
| dlE106 | 0/3 | 0/3 | 0/3 | |
| pmE109 | 2/2 | 1/2 | 2/2 | |
| pmE112 | 3/7 | 2/7 | 2/7 | |
| Total | 8/19 (42) | 6/19 (32) | 6/19 (32) | |
| 1 | wt | 2/5 | 0/5 | 1/5 |
| dlE102 | 4/4 | 4/4 | 3/4 | |
| dlE105 | 4/5 | 5/5 | 2/5 | |
| dlE106 | 4/4 | 3/4 | 1/4 | |
| pmE109 | 4/4 | 3/4 | 0/4 | |
| pmE112 | 4/5 | 2/5 | 3/5 | |
| Total | 22/27 (81) | 17/27 (63) | 10/27 (37) | |
| 2 | wt | 0/5 | 0/5 | 0/5 |
| dlE102 | 3/5 | 1/5 | 1/5 | |
| dlE105 | 3/5 | 3/5 | 2/5 | |
| dlE106b | 4/5 | 4/5 | 1/5 | |
| pmE109 | 4/5 | 2/5 | 0/5 | |
| pmE112b | 2/5 | 2/5 | 2/5 | |
| Total | 16/30 (53) | 12/30 (40) | 6/30 (20) | |
| 3 | wt | 1/5 | 0/5 | 1/5 |
| dlE102 | 0/5 | 2/5 | 0/5 | |
| dlE105 | 1/5 | 2/5 | 0/5 | |
| dlE106 | 2/5 | 0/5 | 2/5 | |
| pmE109 | 4/5 | 1/5 | 1/5 | |
| pmE112 | 4/5 | 0/5 | 0/5 | |
| Total | 12/30 (40) | 5/30 (17) | 4/30 (13) | |
a
DNA was extracted from organs and used for PCR analysis with MAV-1 specific primers. ND, not determined.
b
Urine pool from this group of mice was positive by immunocapture PCR.