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. 2024 Apr 17;14:8914. doi: 10.1038/s41598-024-59604-4

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Temperature-scan experiments of FUS-R495X and -WT. (a) Changes in absorbance (i.e., turbidity) for FUS-R495X as temperature decreases from 38 to 10 °C at 1 bar. (b) Changes in absorbance for FUS-WT as temperature decreases from 38 to 10 °C at 1 bar. (c) The cloud points of FUS-WT (open circles) and FUS-R495X (closed circles) at different protein concentrations at 1 bar. Mean and deviation of the cloud points are shown in the panel. Temperature-scan experiments were performed three times for R495X and five times for WT. Representative data sets are shown in the panels (a) and (b).