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. 2024 Apr 17;14:8902. doi: 10.1038/s41598-024-58536-3

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Top 12 regulated miRNAs in CRC compared with control. (A–F) miRNAs let-7a-5p, let-7c-5p, let-7f-5p, miR-423-5p, and miR-3184-5p, respectively, showed a high level of expression only in CRC stages II. The two lowest expressed in CRC. (G) miRNA miR-99B-5p was observed at low level of expression only in CRC stage IV compared with healthy control. (H) miRNAs miR-30a-5p was observed at low level of expression in CRC stages II. (I) miR-584-5p was up-regulated at CRC stages II and III. (J) miR-204-5p was down-regulated at CRC stages II and IV. (K, L) miR-150-5p and miR-26b-5p were down-regulated at CRC stages II and III. TPM; transcript per million. Red box: up-regulated miRNAs; blue box: down-regulated miRNAs; black box: miRNAs not differentially expressed.