Figure 5.

TLF-II affects the TLR4- NF-κB pathway. (A) Immunohistochemical staining of mouse bladders showing TLR4 immunodetection (brown) in BECs (Scale bar = 50 μm). (B) Western blot analysis of both phosphorylated and total NF-κB. (C) Quantitative analysis of the positive intensity of TLR4 in BECs. (D) Quantification of protein bands for phosphorylated NF-κB with total NF-κB as a control. Data are represented as mean ± SEM of three independent experiments. Significance is indicated as the p value, ##p < 0.01, ###p < 0.001 vs. NC group; *p < 0.05, ***p < 0.001 vs. UTI group.