FIGURE 3.

hnRNPA2B1 contributed to the chemoresistance of GC cells in vivo. (A) SGC7901^VCR shNC and SGC7901^VCR shA2B1 cells were subcutaneously implanted into nude mice, which were then treated with 5‐Fu or saline (20 mg/kg, i.p. injection). The tumor volumes and tumor weight were monitored, and growth curves were plotted every 3 days. (B) Ki‐67 and cleaved Caspase3 staining and percentages in the subcutaneous tumors of SGC7901^VCR shNC and SGC7901^VCR shA2B1 cells. (C) SGC7901 lv‐NC and SGC7901 lv‐A2B1 cells were subcutaneously implanted into nude mice, which were then treated with 5‐Fu or saline (20 mg/kg, i.p. injection). The tumor volumes and tumor weight were monitored, and growth curves were plotted every 3 days. (D) Ki‐67 and cleaved Caspase3 staining and percentages in the subcutaneous tumors of SGC7901 lv‐NC and SGC7901 lv‐A2B1 cells. The data are presented as the mean ± SEM, *P < 0.05, **P < 0.01, *** P < 0.001, ns, not significant by repeated‐measures ANOVA (A and C) or Student's t test (B and D). Scale bars, 50 µm. Abbreviations: hnRNPA2B1, heterogeneous nuclear ribonucleoprotein A2B1; i.p., intraperitoneal; lv‐NC, empty overexpression control; lv‐A2B1, overexpression of hnRNPA2B1; shA2B1, short hairpin RNA of hnRNPA2B1; shNC, negative control short hairpin RNA.