FIGURE 6.

hnRNPA2B1 increased the self‐renewal and tumorigenic capacities of GCSCs in vitro. (A‐B) Representative images (A) and quantification (B) of the sphere‐forming abilities of SGC7901^ADR, SGC7901^VCR and SGC7901 at the indicated passages. Scale bar, 200 µm. (C) Representative images of CD133 /hnRNPA2B1 (left) and CD44/hnRNPA2B1 (right) immunostaining in SGC7901^ADR, SGC7901^VCR and SGC7901 tumor spheroids. Scale bars, 50 µm. The P value was calculated by one‐way ANOVA test (B left and middle) and paired t test (B right). *P < 0.05, ** P < 0.01, *** P < 0.001. Abbreviations: A2B1, heterogeneous nuclear ribonucleoprotein A2B1; CD133, cluster of differentiation 133; CD44, cluster of differentiation 44; GCSC, gastric cancer stem cell; lv‐NC, empty overexpression control; lv‐A2B1, overexpression of hnRNPA2B1; shNC, negative control short hairpin RNA; shA2B1, short hairpin RNA of hnRNPA2B1.