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. Author manuscript; available in PMC: 2024 Apr 18.
Published in final edited form as: Nat Chem Biol. 2023 Jul 27;20(2):151–161. doi: 10.1038/s41589-023-01390-7

Figure 4. Single-cell optical readouts of GPCR-dependent transcriptional signaling.

Figure 4.

A. Representative images of NR4A1 mRNA expression by PLISH analysis. Left: untreated; right: treated with 1μM isoproterenol for 2 h. B. Quantification of NR4A1 induction in cells pre-treated with DMSO (“Vehicle”) or 30μM Dyngo-4A for 20 min, then stimulated with 1μM isoproterenol for 2 h. Data are mean from n = 4 biologically independent replicates ± s.e.m.; 132 cells total/condition. C. Representative images of CREB reporter expression in pCRE-NLS-DD-zsGreen1 cells. Schematic of the reporter is shown on top. CRE = cAMP response element; NLS = nuclear localization sequence. Left: untreated in the presence of 1μM Shield-1 for 4 h; right: treated with 1μM isoproterenol in the presence of 1μM Shield for 4 h. D. Quantification of CREB reporter induction in cells pre-treated with DMSO (“Vehicle”) or 30μM Dyngo-4A for 20 min, then stimulated with 1μM isoproterenol for 4 h in the presence of 1μM Shield. Data are mean from n = 3 biologically independent replicates ± s.e.m.; 225 cells total/condition. **** = p < 0.0001 by two-way ANOVA test with Tukey. Scale bar = 10μm.