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. 2023 Nov 10;36(3):626–641. doi: 10.1093/plcell/koad285

Figure 4.

Figure 4.

TET8 and GIPCs together regulate the release of plant exosomes and EVs. A) TEM observation of EVs in WT, tet8, 35S:IPUT1 in WT (A), IPUT1-RNAi in WT (B), 35S:IPUT1 in tet8 (C), S-TET8 (D), S-CKO (E), S-PCKO (F), 35S:γ2-COPI in WT (G), 35S:γ2-COPI in tet8 (H), γ2-COPI-RNAi in WT (I), and γ2-COPI-RNAi in tet8 (J). CW, cell wall; EVs, extracellular vesicles (black arrows); PM, plasma membrane (white arrows). Scale bars, 500 nm. B) NTA of EVs isolated from the above plants. The value of WT EVs was set to 1.0. Three independent repeats (black dots) were conducted. Statistical significance was determined using Student's t test. *P < 0.05; **P < 0.01; ***P < 0.001.