Figure 7.

Involvement of sirtuins in the beneficial effect of Npre supplementation on spermatogenesis of BU-treated mice. (A) Box plots of FPKM evaluation of Sirt1-7 expression and RT-qPCR of mRNA amount of Sirt2, Sirt3, Sirt4 and Sirt7 in testes of the indicated groups. (B) Left, representative IF for MVH and SIRT2 in sections of seminiferous tubules of testes of the indicated groups (white arrow, SIRT2 located in the nucleus, nuclei counterstained with Hoechst 33342); Right, mean fluorescence intensity of the SIRT2 cytoplasm staining measured in single germ cells. (C) Representative WB and quantitative evaluation of SIRT2 expression relative to GAPDH in testes of the indicated groups. Each result of testicular section was derived from at least 3 to 6 mice. The results include three independent replicates and are showed as mean ± SD (^*P < 0.05; ^**P < 0.01; ns=not significant).