Figure 8.

Inhibition of SIRT2 lessens the beneficial effect of Npre supplementation on ferroptosis and spermatogenesis of BU-treated mice. AGK2 injection (SIRT2 inhibitor) reduces the SIRT2 protein level (A) and counteracts the effect of Npre on number and motility (B) of sperm of BU-treated males (a+b are sperm moving forward). (C) AGK2 resets the level of the ferroptosis marker MDA in the BU+Npre testes nearly to that found in BU-treated mice. (D) Representative WB and quantification levels of GPX4, FTH1 and MVH, relative to GAPDH, in the testes of the indicated groups. (E) Representative protein bands and their quantitative expression of PGC-1α and ACLY in testes from the indicated groups relative to GAPDH. Testis samples were taken from at least 3 to 6 mice in each group, and the results include three independent replicates and are showed as mean ± SD (*P < 0.05; **P < 0.01; ns=not significant).