TABLE 3.
Percent contribution of P450 enzymes to 7-hydroxy-CBD formation
| Formation Rate | Percentage of vehicle control | Est. % Contributiona | Relative % Contributionb | |
|---|---|---|---|---|
| pmol/min/mg protein | % | |||
| Vehicle (control) | 18.81 | 100 | ||
| Sulfaphenazole (CYP2C9) | 12.12 | 64 | 36 | 31 |
| Benzylnirvanol (CYP2C19) | 4.013 | 21 | 79 | 69 |
| Ketoconazole (CYP3A) | 39.23 | 209 | ||
| CYP3cide (CYP3A4) | 22.84 | 121 | ||
aPercent contributions of cytochrome P450 enzymes to 7-OH-CBD formation were estimated by calculating the percent change in the slopes of 7-OH-CBD generation vs. time in reactions with cytochrome P450–selective inhibitors compared with vehicle control.
bBecause the total estimated % contribution was greater than 100%, the data were normalized to 100%, and the same proportional contribution by each cytochrome P450 was retained, according to the method described by Zientek et al. (2016).