TABLE 4.
Percent contribution of P450 enzymes to 7-hydroxy-CBD depletion
Percent contributions of cytochrome P450 enzymes to 7-OH-CBD depletion were estimated by calculating the percent change in the slope of remaining 7-OH-CBD vs. time in reactions with cytochrome P450–selective inhibitors compared with vehicle control. 7-OH-CBD was quantitated using a standard curve (range = 1–1000 ng/ml; limit of quantitation = 5 ng/ml for 7-OH-CBD).
| Clint | Percentage of vehicle control | Est. % Contribution | |
|---|---|---|---|
| µl/min/mg protein | % | ||
| Vehicle (control) | 259.7 | 100 | |
| Benzylnirvanol (CYP2C19) | 233.8 | 90 | 10 |
| Quinidine (CYP2D6) | 300.6 | 116 | |
| Ketoconazole (CYP3A) | 91.75 | 35 | 65 |
| CYP3cide (CYP3A4) | 14.86 | 6 | |