Fig 1.
Validated and predicted xnm5(s2)U34 synthesis pathways in the two main model bacteria. (A) Wobble position of tRNAs. (B) Validated xnm5(s2)U34 synthesis enzymes for E. coli K12. (C) Validated xnm5(s2)U34 synthesis enzymes and pathway holes for B. subtilis 168, S. mutans, and S. pneumoniae. Abbreviations: 5-carboxymethylaminomethyl-2-thiouridine (cmnm5s2U); 5-carboxymethylaminomethyl-2′-O-methyluridine (cmnm5Um); 5-aminomethyl-2-thiouridine (nm5s2U); 5-methylaminomethyluridine (mnm5U); 5-methylaminomethyl-2-thiouridine(mnm5s2U).