TABLE 3.
GSH-dependent formaldehyde dehydrogenase activities
| GSH-dependent formaldehyde dehydrogenase (μ mol NADH/min/mg protein)a | ||||||
|---|---|---|---|---|---|---|
| Wild type | ΔflhS | ΔflhR | ΔgbcAB | ΔhpbC2B2 | ΔgbcAB ΔhpbC2B2 | |
| Succinate | 75 (8) | 6 (2) | 27 (13) | NDb | ND | ND |
| Choline | 361 (107) | 53 (7) | 39 (7) | 95 (35) | 264 (60) | 25 (12) |
| Glycine betaine | 539 (73) | 9 (8) | 21 (12) | 82 (36) | 207 (99) | 26 (13) |
| Dimethylglycine | 313 (62) | 37 (18) | 29 (3) | 244 (106) | 145 (49) | 53 (31) |
| Sarcosine | 135 (39) | 16 (10) | 14 (6) | 140 (90) | 32 (18) | 20 (7) |
| Stachydrine | 63 (23) | 34 (5) | 11 (7) | NDb | ND | ND |
a
GSH-dependent formaldehyde dehydrogenase activities are the means of assays from three independently grown cultures with the standard deviation in parentheses.
b
ND, not done.