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Survival of mice immunized by the gene gun method with pV1J-CDV-H (A) or pV1J-CDV-F (B) after lethal i.c. challenge. Mice were immunized once or twice with pV1J-CDV-H or pV1J-CDV-F at the indicated doses. Controls either were not injected (closed triangles) or were inoculated with blank vector (V1J) (closed circles). All animals were challenged i.c. 2 weeks after the final immunization, and survival was assessed daily for 30 days.
