TABLE 1.
Antibody response in mice immunized with pV1J-CDV-H by different routes
| DNA and route | Dose (μg) | Wk of sampling (no. of inoculations) | Level (mean ± SD; ng/ml) of:
|
|
|---|---|---|---|---|
| IgG1 | IgG2a | |||
| pV1J-CDV-H in PBSa | ||||
| Multipuncture ring | 100 | 7 | 305 ± 169 | 14 ± 7.5 |
| Tail scarification | 100 | 7 | 71 ± 10 | 216 ± 22 |
| Subcutaneous injection | 100 | 7 | —d | — |
| Intramuscular injection | 100 | 6 | 3,300 ± 2,067 | 7,150 ± 4,550 |
| 12 | 7,000 ± 7,970 | 9,000 ± 6,450 | ||
| pV1J-CDV-Hb | ||||
| Gene gun | 2 | 6 (1) | 9,700 ± 1,300 | — |
| 6 (2) | 18,200 ± 7,860 | — | ||
| 27 (1) | 14,000 ± 5,600 | — | ||
| 27 (2) | 26,000 ± 11,900 | — | ||
| 0.5 | 6 (1) | 7,400 ± 800 | — | |
| 6 (2) | 13,500 ± 2,410 | — | ||
| 27 (1) | 7,950 ± 2,000 | — | ||
| 27 (2) | 16,000 ± 1,000 | — | ||
| 0.1 | 6 (1) | 3,400 ± 1,400 | — | |
| 6 (2) | 9,000 ± 3,125 | — | ||
| 27 (1) | 2,200 ± 2,400 | — | ||
| 27 (2) | 10,500 ± 5,300 | — | ||
| pV1Jc | ||||
| Gene gun | 2 | 3 | — | — |
| 7 | — | — | ||
a
Groups of five mice were inoculated with the indicated quantity of DNA in PBS by different methods and routes of immunization.
b
Groups of eight mice were inoculated by the gene gun method with the indicated amounts of DNA. In each group, half of the mice received boosters 3 weeks after the first immunization.
c
Control mice were inoculated by the gene gun method with 2 μg of blank vector.
d
—, level of <1 ng/ml.