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. 2024 Apr 18;19(4):e0298080. doi: 10.1371/journal.pone.0298080

Fig 2. Validation of representative B cell clones confirms their specificity for pS409/410-TDP-43.

Fig 2

(A) Dot blot analysis of rTDP43 treated with or without CK1 using the indicated supernatants from B cell clones. (B) Immunoblot analysis of HEK293T cell lysates expressing GFP, GFP-TDP-25, or GFP-TDP-25mut (S409A/S410A) using the indicated supernatants from B cell clones. GAPDH was used as a loading control. (C) Representative images of immunohistochemical analysis using the supernatants from indicated B cell clones in the hippocampus of human FTLD patients. Arrows mark TDP-43 inclusions. Scale bars are 20 μm.