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T. cruzi CLBrener Luc epimastigotes were incubated for 1 h with 1, 2 and 5% Virkon 1:1 (dashed lines) and 9:1 (solid lines) volumetric excess giving a final Virkon concentration range of 0.5–4.5%. Cultures were monitored for parasite growth over 28 days (three technical replicates, mean ± standard deviation, limit of quantitation 2e4 ml-1). PBS controls were included.
