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. 2024 Jan 4;31(4):610–620. doi: 10.1038/s41594-023-01175-5

Extended Data Fig. 1. Complex preparation and cryo-EM data collection.

Extended Data Fig. 1

a Elution curve of CXCR3-CXCL11-DNGi-scFv16 complex on Superose 6 Increase column. b SDS-PAGE of CXCR3-CXCL11-DNGi-scFv16 complex after size-exclusion chromatography. c Cryo-EM image of CXCR3-CXCL11-DNGi-scFv16 particles (a representative of 5,500 micrographs). d Elution curve of CXCR3-PS372424-DNGi-scFv16 complex on Superose 6 Increase column. e SDS-PAGE of CXCR3-PS372424-DNGi-scFv16 complex after size-exclusion chromatography. f Cryo-EM image of CXCR3-PS372424-DNGi-scFv16 particles (a representative of 3,185 micrographs). g Elution curve of CXCR3-VUF11222-DNGi-scFv16 complex on Superose 6 Increase column. h SDS-PAGE of CXCR3-VUF11222-DNGi-scFv16 complex after size-exclusion chromatography. i Cryo-EM image of CXCR3-VUF11222-DNGi-scFv16 particles (a representative of 2,891 micrographs). j Elution curve of CXCR3κOR-SCH546738-Nb6 complex on Superose 6 Increase column. k SDS-PAGE of CXCR3κOR-SCH546738-Nb6 complex after size-exclusion chromatography. l Cryo-EM image of CXCR3κOR-SCH546738-Nb6 particles (a representative of 12,944 micrographs). In b, e, h, and k, data shown are representative of two independent experiments.

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