FIG. 3.

Photographic image of HeLa cell proteins, electrophoretically separated in denaturing gels and reacted with a preimmune serum (lane 1) or with a serum to p78 (lane 2). Uninfected HeLa cell extract was separated in a 10% denaturing polyacrylamide gel, blocked, reacted with a rabbit preimmune serum or serum to p78 (see Materials and Methods) and then with a goat anti-rabbit antibody conjugated to alkaline phosphatase, and processed as described by the manufacturer (Bio-Rad). The major p78 band resolves as a doublet in lower-percentage polyacrylamide gels.