Table 1.
Frequencies of T, B, and NK subpopulations in lymphocytes from control individuals and the heart-grafted patienta
| T cells (CD3+) | B cells (CD19+) | NK cells | |||||
|---|---|---|---|---|---|---|---|
| CD4+ | CD4+CD25highFoxp3+ | CD8+ | CD24high | CD38+ | CD3−CD56+ | ||
| Controls (n = 7) | |||||||
| Mean | 35.9b | 1.8 | 13.0 | 10.9 | 2.1 | 8.7 | 26.1 |
| SDc | 14.0 | 1.0 | 5.0 | 4.6 | 1.0 | 3.1 | 18.2 |
| Patient | 34.6 | 1.0 | 33.9 | 3.6 | 0.7 | 3.2 | 4.5 |
aPBMC were isolated by Ficoll-gradient centrifugation, stained with appropriate antibody combinations and analyzed on a FACSCalibur flow cytometer
bResults are expressed as % positive cells among “gated” lymphocytes. For controls, mean values and standard deviations are given as obtained by the analysis of samples from 7 healthy individuals (mean age 68.8 ± 5.5 years). Patient data represent mean values obtained from quadruple measurements
cStandard deviation