Figure 4.

Increased GIRK2 expression counteracts hyperactivity induced by low-ethanol conditions. A, Timeline of neuronal differentiation, GIRK2 upregulation, and ethanol exposure. B, Schematic of 7 d IEE, showing change in ethanol concentration over 24 h due to evaporation (Scarnati et al., 2020; Popova et al., 2023). C–E, Neuronal firing rates (WMFR) measured with MEA is shown for NGN2 neurons at Days 28 (1 week ethanol; C), 35 (2 weeks ethanol; D), and 42 (3 weeks ethanol; E); each point represents an MEA well; N = 2 donors|5 batches. Point color indicates GIRK2 expression method (coral, CRISPRa; teal, Lenti). GLMM includes batch and donor random effects. *p < 0.05.