Fig. 6. Read coverage analyses with RNA-seq data suggest that low-complexity regions (LCRs) 3 and 21 are transcribed during monkeypox virus (MPXV) infection.

a–g The positional depth of translation starts, panning from the 40 nucleotides upstream to the 10 nucleotides downstream of the first alternative start codon (ATG), of top early genes. The vertical blue line represents the first ATG of the gene, and the arrow shows the spans of the first 10 (canonical) coding nucleotides. g, h If present, red lines represent ATGs, evidenced from transcriptomic data demonstrating that both OPG204 and OPG208 ATGs, located upstream of the canonical ones, are transcribed during MPXV subclade IIb infection. ORF, open reading frame.