FIG. 5.

Viral RNA and protein expression levels in MEFs derived from WT (STAT+/+) and STAT−/− CD1 mice. (A) STAT+/+ and STAT−/− MEFs were infected with WSN virus at an MOI of 5, and vRNA levels specific for the NA and NS genes were determined by PAGE analysis of primer extension products at different times p.i. (B) STAT+/+ and STAT−/− MEFs were infected with WSN virus at an MOI of 2 and ³⁵S labeled at the indicated time points, and total amount of viral proteins was immunoprecipitated with a polyclonal antiserum against WSN virus. Immunoprecipitated products were analyzed by SDS-PAGE.