TABLE 2.
WSN virus growth determined by HA assay
Fibroblast culture | Virus titera
|
|||
---|---|---|---|---|
48 h | 60 h | 72 h | 84 h | |
WT cell line | <2 | <2 | <2 | <2 |
WT MEFs | <2 | <2 | <2 | <2 |
STAT1−/− cell line | <2 | 8 | 16 | 32 |
STAT1−/− MEFs | 16 | 32 | 64 | ND |
IFNγ−/− MEFs | <2 | <2 | <2 | <2 |
IFNαR−/− MEFs | 32 | 64 | 64 | 64 |
Cell monolayers were infected with WSN virus at an MOI of 0.001 and incubated at 37°C. At the indicated time points, the amount of virus in the supernatant was determined by HA assay. At 12, 24, and 36 h p.i., titers in all cultures were <2. ND, not done.