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Immunofluorescence assay showing the ability of BBLF4, BSLF1, and BBLF2/3 to form a tripartite complex that localizes to the nucleus in cotransfected Vero cells. Cotransfection of any two of the helicase-primase proteins did not confer nuclear localization. Transfected proteins were visualized with anti-Myc antibody and FITC-conjugated anti-mouse IgG secondary antibody.
