TABLE 2.
Effect of Zta activation domain deletions on localization of BBLF4 and BBLF2/3 plus BSLF1
| Localization (% of positive cells)a
|
|||
|---|---|---|---|
| Nucleus | Nucleus + cytoplasm | Cytoplasm | |
| Myc-BBLF2/3 + BSLF1 | 0 | 0 | 100 |
| +wt Zta | 61 ± 2 | 28 ± 1 | 11 ± 2 |
| +Zta(Δ2-25) | 7 ± 4 | 5 ± 5 | 88 ± 2 |
| +Zta(Δ13-19) | 2 ± 2 | 1 ± 1 | 97 ± 3 |
| Myc-BBLF4 | 0 | 0 | 100 |
| +wt Zta | 91 ± 5 | 9 ± 5 | 0 |
| +Zta(Δ2-25) | 91 ± 1 | 9 ± 1 | 0 |
| +Zta(Δ13-19) | 61 ± 1 | 30 ± 1 | 9 ± 1 |
a
Myc-tagged proteins were detected in transfected Vero cells by indirect immunofluorescence using anti-Myc antibody and FITC-conjugated anti-mouse Ig. Values are averages of two experiments.