FIG. 2.

(A) Schematic of full-length and truncated forms of ς1s expressed as fusion proteins with MBP. Sequences of the T3D ς1s ORF were cloned into the pMAL-c2 vector. MBP fusion proteins containing β-galactosidase, full-length ς1s (MBP-ς1s/1–120), or truncations of ς1s (MBP-ς1s/1–84 and MBP-ς1s/1–42) were expressed in E. coli and purified by affinity chromatography using an amylose resin. (B) Immunoblot of MBP-ς1s fusion proteins using MAbs 2F4 and 3E2. The upper gel shows the MBP-β-galactosidase fusion protein as a control and the three MBP-ς1s fusion proteins after electrophoresis in a 10% polyacrylamide gel and staining with Coomassie blue (1 μg of protein per lane). The lower two gels are immunoblots of the same four proteins (20 ng of protein per lane) using MAbs 2F4 and 3E2 (5 μg per ml). Molecular size markers are given in kilodaltons.