Figure 6.

Selective labeling of Kme₂ proteins by TACO chemistry using varying fluorophores. (a) Selective modification of chemically generated Kme₂ proteins to aldehyde proteins by TACO chemistry followed by labeling with varying fluorophores using different aldehyde chemistries as analyzed by running SDS PAGE. No fluorophore labeling was observed without the TACO reaction. (b) Selective modification of chemically generated Kme₂ proteins in cell lysate to aldehyde proteins by TACO followed by fluorophore labeling and in-gel analysis. High fluorescence was observed in cell lysate in a dose-dependent manner, indicating high amounts of Kme₂-proteins. No fluorophore labeling was observed on cell lysate without the TACO reaction or without Kme₂-modified cell lysate.