Fig. 8.

Expression of Glb1l4 in rat epididymis during the entire life span in days (D). A) Northern blot analysis for Glb1l4 mRNA and 18s rRNA from the caput region (1), the corpus region (2), and the cauda region (3) during development. B) Western blot analysis of GLB1L4 and ACTIN proteins during development. D, days. C) Quantitative analysis of the relative mRNA and protein expression levels in rat epididymides at different ages. RNA and protein were pooled from three animals per group. D) Left: RT-PCR of two isoforms of Glb1l4 mRNA expression in rat caput epididymis from 30, 45, 60, and 120 days (D) after birth. Gapdh was used as internal control. NC, negative control. Right: semiquantitative analysis of the ratio of isoform2:isoform1 in rat epididymis at different ages. d, days.