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hCrm1 is a nucleocytoplasmic shuttle protein. Recombinant purified GST-Crm1 was mixed with a rabbit IgG tracer and then injected into one nucleus of a binuclear HeLa cell. While the IgG tracer remains behind (A), the Crm1 fusion protein relocalizes to the other nucleus, and in particular to the nuclear membrane, as well as to the cytoplasm (B).
