Fig. 3.

The effect of the vaccine on immunosuppressive cells in the TME. The vaccine reduced the number of intratumoral immunosuppressive cells, including a, b MDSCs; c, d Tregs; e, g M2-TAMs. Representative flow cytometry scatter plots from one mouse (one out of three mice) showing intratumoral a MDSCs, c Tregs, e M1-TAMs, and M2-TAMs. Flow cytometry data showing tumor-infiltrating MDSCs, Tregs, M1-TAMs and M2-TAMs is represented as a bar graph expressed as b %CD11b+ Gr-1+ cells, d %CD4+ Foxp3+ cells, f %CD11b+ F4/80+ CD86+ cells and g %CD11b+ F4/80+ CD206+ cells. The data are depicted as the mean ± SD (n = 3). The significance of the data was evaluated by one-way ANOVA followed by Tukey’s multiple comparison test (*p < 0.05, **p < 0.01)