TABLE 1.

Amplimers used

Primer	Sequence (5′-3′)a	Restriction site	Positionb	Gene
2190	AGCACTGCAAGCTTGAACTA	HindIII	−7	TGEV M
2282	GTTGGCGAATTCGAAGTTTAGTTATACCATATG	EcoRI	778	TGEV M
3247	CTAAACACCATGGCGATTTTGTTAATATTAGCGTGTG	NcoI	28	TGEV M
2414	GAAGAAGGGATCCATAACTATGGCGTTTCC	BamHI	11	TGEV E
2030	TTGTCTAGATCAAGCAAGGAGTG	XbaI	235	TGEV E
66830	CCGATTCCATGGCTAGTGTAACTACACCAGCACCA	NcoI	27	BCV M
66841	AGGGATCCTTAAAGTTTAGATATTATTTCTC	BamHI	678	BVC M
627	ATGTTTATGGCTGATGC		17	BCV E
519	GTAAACTGGTGCTGGTGTAGTTAC		262	BCV E
2373	ATAGACCAGCTGAAGTTCCATTCCTTTAGGAA		61	BCV M
72916	GGAACTTCAGCTGGTCTATAA		181	TGEV M
85138	TCCAGTTCGCGAGATGCCAAATAAGATCGCC		133	TGEV M
85139	TTGGCATCTCGCGAACTGGAACTTTTCTTTGGGTATTATAC		97	BCV M
2119	TAATACGACTCACTATAGG			T7 promoter
85137	AGTTGAATGCATAATTTAAAAGTTGCCAAAAATGCC	NsiI	98	BCV E
85136	TTAAATTATGCATTCAACTTTGCGGTATGTG	NsiI	145	BCV E

^aOverlapping regions for primer pairs 2373-72916 and 85138-85139 are in boldface; restriction sites are underlined. 

^bPosition of the last nucleotide of the primer relative to that of the first coding nucleotide.