Fig. 2.

Characterization of IBI101 in the in vitro cell-based assays. a The Jurkat-OX40 reporter cells co-cultured with Raji cells were stimulated with anti-CD3/CD28 and anti-OX40 antibodies, followed by luciferase activity determination. b The Jurkat-OX40 reporter cells without Raji cells were stimulated with anti-CD3/CD28 and anti-OX40 antibodies, followed by luciferase activity determination. c The Jurkat-OX40 reporter cells were stimulated with anti-CD3/CD28 and 100 nM OX40L in the presence of indicated anti-OX40 antibodies, followed by luciferase activity determination. d Human CD4+ T cells were activated by plate-bound anti-CD3 and anti-OX40. Then IL-2 secretion was determined by ELISA. e Effect of anti-OX40 mAbs on T cell proliferation. Far Red fluorescent-labeled PBMCs were cultured in 96-well plate coated with anti-CD3 and anti-OX40 mAbs or h-IgG for four days. The proliferation of CD8 and CD4 T cells was determined by flow cytometry. POGA means the reference antibody pogalizumab