Fig. 2.

Tumor-associated macrophage and microglial cells are decreased by treatment with NE-siRNA CD73. Rats with implanted GB were treated with NE-siRNA CD73 as described in Fig. 1. After tumor dissociation, the frequencies of macrophages, microglia, and activated alternative phenotypes were determined by immunostaining, followed by flow cytometry. The percentages of a microglia (CD11b⁺CD45^low), b macrophages (CD11b⁺CD45^high), c M2-like microglia (CD11b⁺CD45^lowCD206⁺), and d M2-like macrophages (CD11b⁺CD45^highCD206⁺) were determined. The values represent the mean ± SEM from five animals. *indicates a significant difference from the GB control group, as determined by a Student’s t test. NE-siRNA CD73: siRNA CD73-loaded cationic nanoemulsion; GB: glioblastoma