Fig. 5.

CD73 expression is selectively downregulated in glioblastoma-associated macrophages and microglial cells following NE-siRNA CD73 therapy. Rats with implanted GB were treated with NE-siRNA CD73 as described in Fig. 1. After tumor dissociation, CD73 expression was determined by immunostaining, followed by flow cytometry. CD73 expression was analyzed using a gating strategy that identified the cells of the glioblastoma microenvironment. CD73 expression was also analyzed in a tumor tissues, b CD11b⁺CD45^low microglia, c CD11b⁺CD45^high macrophages, and d CD4⁺, e CD4⁺CD25^low, and f CD4⁺CD25^high lymphocytes. The values represent the mean ± SEM from five animals. *indicates a significant difference from the GB control group, as determined by a Student’s t test. NE-siRNA CD73: siRNA CD73-loaded cationic nanoemulsion; GB: glioblastoma