FIG. 1.

Virus isolated from CD4⁺ T lymphocytes of donor 6 grows slowly in culture relative to that of other HIV-infected individuals. CD4⁺ lymphocytes from HIV-infected donors were cocultured with a pool of allogeneic CD4⁺ T cells and sampled at the times indicated. CD4⁺ lymphocytes were selected onto AIS flasks coated with anti-CD4 antibodies. Thirty million PBMC were added to each flask, and after a 1-h incubation, the nonadherent cells were removed. Seven million allogeneic CD4⁺ lymphocytes activated with anti-CD3 antibody (45) were added to the adherent cell population, and the cultures were maintained in the presence of 5,000 U of interleukin-2 (Chiron)/ml for 21 to 28 days. Culture supernatants were sampled every 7 days, at which time fresh medium was added to the cultures. (A) CD4⁺ lymphocytes from eight HIV-infected individuals. Donors are as indicated on the figure. Donor 13, a rapid progressor to disease, is included for the purposes of comparison. (B) CD4⁺ lymphocytes from donor 6 were tested on four different occasions.