FIG. 4.

Recombinant viruses are capable of replicating in M∅. Infections were performed in 96-well microtiter plates. For infections, 2 × 10⁵ cells were incubated with virus made by electroporation of PBMC for 2 h as described in Materials and Methods. M∅ from three donors were prepared, and the cells were pooled and plated for infection on day 6. Infections were performed on 7-day-old M∅. The M∅ donors were different from the CD4⁺ T-cell donors in Fig. 3. Clones are as indicated. Data points represent the averages of at least two independent experiments. If error bars are drawn, data points represent the averages of three independent experiments.