Fig. 4.

CD8⁺ T cell dysfunction in diffuse type gastric cancer correlates with the lavish expression of immunosuppressive factors. a mRNA expression of immunosuppressive factors by different Lauren subtypes of gastric cancer in the TCGA cohort (n = 235). b Expression of IL-10, TGF-β1 and IDO1 in gastric cancer tissues with different Lauren subtypes detected by immunohistochemistry in Zhongshan TMA cohort (n = 456). c Expression of IL-10, TGF-β1 and IDO1 in gastric cancer tissues with different Lauren subtypes detected by immunohistochemistry in Zhongshan FCM cohort (n = 43). d A 2D heatmap showing different expressions of immunosuppressive factors and CD8⁺ T cell functional status in Zhongshan FCM cohort (n = 43). Colors show row-normalized z-score of immunosuppressive factor expression scores or cell populations percentages. e Association between expression level of immunosuppressive factors and functional status of tumor-infiltrating CD8⁺ T cells. Data are represented as mean ± SD and P values are calculated using unpaired two-tailed t test. IL-10 interleukin-10, TGF-β1 transforming growth factor-β1, TMA tissue microarray, FCM flow cytometry, n.s no significance